If I only have regular sized muffin trays, could I just make a bigger puff (4")?

Hi Sara and Michael! All the years my family has been making these, we've never tried a bigger puff—sounds fun! If you increased the puff pastry square size, stuffed it with more filling, and lengthened the bake time, I don't see why that wouldn't work. (I'd try that versus putting a small puff into a larger muffin cup—worried it might fall apart.) Let me know how it turns out if you give it a go!